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- 品牌：Biotoolomics
- 型號(hào)：5 ml;50 ml;1 L
- 貨號(hào)：380201
- 發(fā)布日期： 2023-03-03
- 更新日期： 2024-08-12
| 分辨率 | 詳詢 |
| 重量 | 詳詢 |
| 品牌 | Biotoolomics |
| 貨號(hào) | 380201 |
| 電源電壓 | 詳詢 |
| 型號(hào) | 380201 |
| 測(cè)量范圍 | 詳詢 |
| 規(guī)格 | 5 ml;50 ml;1 L |
| 加工定制 | 是 |
| 外形尺寸 | 詳詢 |
| 測(cè)量精度 | 詳詢 |
Product Introduction:
Carboxyl-activated SepFast MAG is used to make magnetic chromatography media. It forms chemically stable amide linkages with ligands containing primary amine groups. It has a long spacer (9 atoms). After binding of target molecules, the magnetic resin can be readily isolated from the biological feedstocks with the aid of a magnet. Carboxyl-activated agarose can be used for the immobilization of amine containing molecules. The coupling chemistry is carbodiimide-based and is a successful and well-documented technique.
Product Properties:
Carboxyl-activated SepFast MAG 4HF is made of highly cross-linked 4% agarose containing magnetic material. It makes harsher operational conditions possible so that low pH and certain organic solvents of high concentrations can be applied. Agarose has long been used for chromatographic separations due to its excellent hydrophilic and low non-specific-binding nature. The particles have an open pore structure with excellent mass transfer properties to large protein molecules. The base matrix is activated by attaching a long hydrophilic spacer arm to the backbone. It reacts directly with primary amine groups in the presence of a carbodiimide agent. Carboxyl-activated SepFast MAG is supplied as an aqueous suspension in 20% ethanol.
Compatible with all commonly used aqueous chemicals, provided the ligand
to be coupled can withstand
Group to be coupled
-NH2
Matrix
Highly cross-linked 4% magnetic agarose (4HF)
Particle size*
50 – 150 μm
Spacer arm
9 atoms
Activation level
8 - 19 μmol carboxyl / ml medium
pH stability
4 -13 (ligand dependent)
Chemical stability
Storage
+4℃ - +8℃
