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- 品牌：Invivogen
- 產(chǎn)地：國(guó)外
- 型號(hào)：詳詢
- 貨號(hào)：hkb-kotifa
- 發(fā)布日期： 2023-03-31
- 更新日期： 2024-08-12
| 產(chǎn)地 | 國(guó)外 |
| 保存條件 | -20℃保存 |
| 品牌 | Invivogen |
| 貨號(hào) | hkb-kotifa |
| 用途 | 科研 |
| 組織來(lái)源 | 詳詢 |
| 細(xì)胞形態(tài) | 詳詢 |
| 是否是腫瘤細(xì)胞 | 是 |
| 保質(zhì)期 | 詳詢 |
| 器官來(lái)源 | 詳詢 |
| 免疫類(lèi)型 | 詳詢 |
| 品系 | 詳詢 |
| 生長(zhǎng)狀態(tài) | 詳詢 |
| 物種來(lái)源 | 詳詢 |
| 包裝規(guī)格 | 詳詢 |
| 是否進(jìn)口 | 是 |
Cell Line Stability
Cells will undergo genotypic changes over time resulting in reduced responsiveness in normal cell culture conditions. Genetic instability is a biological phenomenon that occurs in all stably transfected cells. Therefore, it is critical to prepare an adequate number of frozen stocks at early passages. HEK-Blue™ KO-TIFA cells should not be passaged more than 20 times to remain fully functional.
Quality Control
? TIFA gene knockout has been verified by DNA sequencing, RT-qPCR, and functional assays.
? The stability for 20 passages following thawing has been verified.
? These cells are guaranteed mycoplasma-free.
CELL LINE DESCRIPTION
HEK-Blue™ KO-TIFA cells were generated from HEK-Blue™ Null1-v cells through the stable knockout of the TIFA gene. The parental cells derive from human embryonic kidney 293 (HEK-293) cells. HEK-Blue™ KO-TIFA cells express a secreted embryonic alkaline phosphatase (SEAP) under the control of an NF-κB-inducible promoter comprised of an IFN-β minimal promoter fused to five NF-κB and AP-1 binding sites. Levels of SEAP in the supernatant can be easily determined with HEK-Blue™ Detection, a SEAP detection cell culture medium. Unlike their parental cell line, HEK?Blue™ KO-TIFA cells do not respond to cytosolic ADP?Heptose. However, they do respond to other NF-κB inducers such as TNF-α and IL-1β. HEK?Blue™ KO-TIFA cells are resistant to Zeocin™
